Article No
RN019M
Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
Antibodies Primary
Specifications
| Application | IP, ICC |
| Article No | RN019M |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | 235-1 |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb |
| Supplier | MBL |
| Isotype | IgG2a k |
| Notes | Small nuclear RNA (snRNA) is known to be involved in pre-mRNA splicing as an RNA component of snRNP. 2,2,7-trimethylated guanosine (m3G/TMG) is a modified nucleoside at the 5’-cap site of U snRNAs except for U6 snRNA. Shortly after transcription, 7-methylguanosine (m7G), called cap structure, is added to 5’-end of newly synthesized U snRNA and m7G-capped U snRNA is exported to the cytoplasm. The m7G-cap is converted to m3G-cap, which is a hypermethylated structure of 5’-end, by cytoplasmic catalytic protein TSG1 and the m3G-capped U snRNA forms snRNP with Sm proteins in the cytoplasm, followed by entry into the nucleus. The m3G-capped snRNP plays a role in regulation of pre-mRNA splicing as a part of spliceosome in nucleus. It is also reported that U3 and U8 snoRNA, localized to Cajal body during RNP biogenesis, possess m3G-cap structure. |
| Alias Names | N2,N2,7-Trimethylguanosine, m2,2,7G |
| Product Type | Antibodies Primary |
| Research area | Epigenetics |
| Shipping Information | -20°C |
| Size | 200 ug/200 uL |
| Source / Host | mouse |
| Storage | -20°C |
| Substrate / Buffer | 1 mg/mL in PBS/50% glycerol, pH 7.2 |
| Technical Specifications | Small nuclear RNA (snRNA) is known to be involved in pre-mRNA splicing as an RNA component of snRNP. 2,2,7-trimethylated guanosine (m3G/TMG) is a modified nucleoside at the 5’-cap site of U snRNAs except for U6 snRNA. Shortly after transcription, 7-methylguanosine (m7G), called cap structure, is added to 5’-end of newly synthesized U snRNA and m7G-capped U snRNA is exported to the cytoplasm. The m7G-cap is converted to m3G-cap, which is a hypermethylated structure of 5’-end, by cytoplasmic catalytic protein TSG1 and the m3G-capped U snRNA forms snRNP with Sm proteins in the cytoplasm, followed by entry into the nucleus. The m3G-capped snRNP plays a role in regulation of pre-mRNA splicing as a part of spliceosome in nucleus. It is also reported that U3 and U8 snoRNA, localized to Cajal body during RNP biogenesis, possess m3G-cap structure. |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
