2,2,7-trimethylguanosine

Article No RN011M
Product Type Antibodies Primary
Research Area Epigenetics
Application ICC, IP, RIA
Size 200 µl

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Specifications

Country Availability SE, FI, DK, NO, IS, EE, LV, LT
Clone C1-36
Clone Type monoclonal
Data Sheet Link https://www.mblintl.com/assets/RN011M-v1.pdf
Description Anti-2,2,7-trimethylguanosine (m<sub>3</sub>G/TMG) mAb
Supplier MBL International Corp.
Immunogen BSA-conjugated trimethylguanosine (m<sub>3</sub>G/TMG)
CE/IVD false
Isotype IgG2b k
Notes Small nuclear RNA (snRNA) is known to be involved in pre-mRNA splicing as an RNA component of snRNP. 2,2,7-trimethylated guanosine (m<sub>3</sub>G/TMG) is a modified nucleoside at the 5&rsquo;-cap site of U snRNAs except for U6 snRNA. Shortly after transcription, 7-methylguanosine (m<sup>7</sup>G), called cap structure, is added to 5&rsquo;-end of newly synthesized U snRNA and m<sup>7</sup>G-capped U snRNA is exported to the cytoplasm. The m<sup>7</sup>G-cap is converted to m<sub>3</sub>G-cap, which is a hypermethylated structure of 5&rsquo;-end, by cytoplasmic catalytic protein TSG1 and the m<sub>3</sub>G-capped U snRNA forms snRNP with Sm proteins in the cytoplasm, followed by entry into the nucleus. The m<sub>3</sub>G-capped snRNP plays a role in regulation of pre-mRNA splicing as a part of spliceosome in nucleus. It is also reported that U3 and U8 snoRNA, localized to Cajal body during RNP biogenesis, possess m<sub>3</sub>G-cap structure.
References 1) Abreu, R. S., et al., J. Biol. Chem. E publication March 3, (2009) 2) Susaki, K., et al., Exp. Eye Res. 88, 347-355 (2009) 3) Kawahara, H., et al., J. Cell Biol. 181, 639-653 (2008) 4) Imai, T., et al., Mol. Cell Biol. 21, 3888-38900 (2001)
Source / Host mouse
Storage -20°C
Substrate / Buffer 1 mg/mL in PBS/50% glycerol, pH 7.2

Shipping info

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Source/Host:

mouse mouse

Product insert:

Product data sheet

Datasheet

Suggested protocols:

Immunoprecipitation

Immunoprecipitation