Article No
PM036-PN
Positive control for anti-LC3 antibody
Positive control for anti-LC3 antibody
Antibodies Primary
Specifications
| Application | WB |
| Article No | PM036-PN |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone Type | polyclonal |
| Components | 1x100000 cells of human LC3B/293T were suspended with 100 uL of Laemmli’s sample buffer. This product contains 5% 2-mercaptoethanol. Handle with care. |
| Conjugation | Unconjugated |
| Description | Positive control for anti-LC3 antibody |
| Supplier | MBL |
| Notes | Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3. |
| Product Type | Antibodies Primary |
| Research area | Cell Death |
| Shipping Information | 4°C |
| Size | 100 µl (10 Tests) |
| Stability | 1 year |
| Storage | -20°C |
| Substrate / Buffer | 1x105 cells of human LC3B/293T were suspended with 100 μl of Laemmli’s sample buffer.This product contains 5% 2-mercaptoethanol. Handle with care. |
| Technical Specifications | Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3. |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
