Article No
PAT-SUA46J-100
Recombinant E.coli Dnak(385-638aa) protein
Recombinant E.coli Dnak(385-638aa) protein
Proteins & Peptides
Specifications
| MW | 27.7 kDa (255aa) confirmed by MALDI-TOF |
| NCBI Number | NP_414555 |
| Accession Number | NP_414555 |
| Application | Electrophoresis |
| Article No | PAT-SUA46J-100 |
| Biosite Brand | Biosite Proteins |
| Country Availability | all |
| Concentration | 1 mg/ml |
| Description | Recombinant E.coli Dnak(385-638aa) protein |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 944750 |
| Gene Symbol | dnaK |
| Notes | DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. The protein coding region of the substrate binding domain of DNAK (amino acids 385-638) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus |
| Alias Names | Dnak (C-term, 385-638), Substrate binding domain, Heat shock protein 70, Heat shock 70 kDa protein, HSP70, Chaperone protein dnaK, Chaperone Hsp 70, Co chaperone with DnaJ, dnaK, Heat shock 70 kDa protein, |
| Previous Article No | PAT-SUA46J-100, SUA46J-100 |
| Product Type | Proteins & Peptides |
| Purity | >95% |
| Sequence | MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQG ERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HSTRKQVEEA GDKLPADDKT AIESALTALE TALKGEDKAA IEAKMQELAQ VSQKLMEIAQ QQHAQQQTAG ADASANNAKD DDVVDAEFEE VKDKK |
| Size | 100ug |
| Species Reactivity | bacteria |
| Storage | 4°C, -80°C |
| Substrate / Buffer | Liquid. 25 mM Tris-HCl, pH7.5, 100 mM NaCl, 5 mM DTT, 10%Glycerol |
| UniProt Number | P0A6Y8 |
| Product Page Updated | 2024-04-17T08:28:29.021Z |