Lysates

293T Lysate

293T Lysate

Article No

PAT-PK1610-200

Application

WB

Species Reactivity

human

Size

200ug

Suggested protocols

Specifications

Application WB
Article No PAT-PK1610-200
BioSite Brand BioSite
Country Availability all
Concentration 2 mg/ml
Description 293T Lysate
Supplier Nordic BioSite
Notes 293T cells are a human embryonic kidney (HEK) cell line that expresses the large T antigen. The large T antigen is commonly used for transfection assays and is required for transient transfection of plasmids with an SV40 origin of replication. 293T cells are able to grow well in reduced serum medium or in medium lacking calcium ions. In addition, 293T cells tend to grow at a faster rate and to a higher cell density than the parent 293 cell line, resulting in increased amounts of endogenously expressed protein.
Previous Article No 209-PK1610-200
Product Type Lysates
Protocol Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Size 200ug
Species Reactivity human
Storage -80°C
Substrate / Buffer Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Technical Specifications Western Blot analyzed by both GAPDH and Beta tubulin antibody
Product Page Updated 2022-04-08T09:23:07.996Z

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