Article No
PAT-I1TFZ5-100
Recombinant e.coli gldA protein
Recombinant e.coli gldA protein
Enzymes
Specifications
| MW | 41.1 kDa (390aa) confirmed by MALDI-TOF |
| NCBI Number | NP_418380 |
| Accession Number | NP_418380 |
| Application | Electrophoresis |
| Article No | PAT-I1TFZ5-100 |
| Biosite Brand | Biosite Proteins |
| Country Availability | all |
| Concentration | 1 mg/ml |
| Description | Recombinant e.coli gldA protein |
| Supplier | Nordic Biosite |
| Entrez Gene ID | 948440 |
| Gene Symbol | gldA |
| Notes | gldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E. coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i. e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1, 2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1, 2-propanediol, respectively. Recombinant E. coli gldA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Alias Names | Glycerol dehydrogenase, ECK3937, JW5556 |
| Previous Article No | PAT-I1TFZ5-100, I1TFZ5-100 |
| Product Type | Enzymes |
| Purity | >95% |
| Sequence | MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE |
| Size | 100ug |
| Species Reactivity | bacteria |
| Storage | 4°C, -80°C |
| Substrate / Buffer | Liquid. In Phosphate buffered saline (pH7.4), 10% glycerol |
| UniProt Number | P0A9S5 |
| Product Page Updated | 2024-04-08T12:54:05.243Z |