ABO

Article No PAT-81225-1
Product Type Proteins & Peptides
Research Area Cell Proliferation
Application Electrophoresis
Size 50ug

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Specifications

MW 37.4kDa (322aa), confirmed by MALDI-TOF
NCBI Number NP_065202
Country Availability SE, NO, DK, FI
BioSite Brand BioSite
Concentration 0.5mg/ml (determined by Bradford assay)
Description ABO, also known as NAGAT, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of uDP-GalNAc) or to the B antigen (by addition of uDP-Gal), whereas O individuals lack such activity. Recombinant human ABO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Supplier BioSite
Keywords Enzymes
Notes ABO, also known as NAGAT, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of uDP-GalNAc) or to the B antigen (by addition of uDP-Gal), whereas O individuals lack such activity. Recombinant human ABO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Alias Names Histo-blood group ABO system transferase, A3GALNT, A3GALT1, GTB, NAGAT, A transferase, B transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, alpha 1-3-N-acetylgalactosaminyltransferase, alpha 1-3-galactosyltransferase
Purity > 85% by SDS - PAGE
Sequence MGSSHHHHHH SSGLVPRGSH MAVREPDHLQ RVSLPRMVYP QPKVLTPCRK DVLVVTPWLA PIVWEGTFNI DILNEQFRLQ NTTIGLTVFA IKKYVAFLKL FLETAEKHFM VGHRVHYYVF TDQPAAVPRV TLGTGRQLSV LEVRAYKRWQ DVSMRRMEMI SDFCERRFLS EVDYLVCVDV DMEFRDHVGV EILTPLFGTL HPGFYGSSRE AFTYERRPQS QAYIPKDEGD FYYLGGFFGG SVQEVQRLTR ACHQAMMVDQ ANGIEAVWHD ESHLNKYLLR HKPTKVLSPE YLWDQQLLGW PAVLRKLRFT AVPKNHQAVR NP
Source / Host bacteria
Species Reactivity human
Storage Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Substrate / Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
UniProt Number P16442
Product Page Updated 2019-04-15T12:57:56.440Z

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Source/Host:

bacteria

Species reactivity:

human human