Proteins & Peptides

14-3-3 epsilon

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The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human 14-3-3 epsilon was expressed in E. coli and purified by using conventional chromatography techniques.

Article No

PAT-80002-1

Application

Electrophoresis

Species Reactivity

human

Size

100ug

Source / Host

bacteria

Specifications

MW 29 kDa (255 aa), confirmed by MALDI-TOF.
NCBI Number NP_006752
Application Electrophoresis
Article No PAT-80002-1
BioSite Brand BioSite
Concentration 1 mg/ml (determined by Bradford assay)
Description The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human 14-3-3 epsilon was expressed in E. coli and purified by using conventional chromatography techniques.
Supplier BioSite
Domain 1-255aa
Keywords Signaling Pathway
Notes The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human 14-3-3 epsilon was expressed in E.coli and purified by using conventional chromatography techniques.
Alias Names Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein epsilon polypeptide, YWHAE, MDS, MDCR, KCIP-1, FLJ45465, 14-3-3 epsilon, Tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, epsilon polypeptide 14 3 3 E, 14 3 3E, KCIP 1, KCIP1, Mitochondrial import stimulation factor L subunit, Protein kinase C inhibitor protein1, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide.
Product Type Proteins & Peptides
Purity > 95% by SDS - PAGE
Research Area Cell Signaling
Sequence MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV EDENQ
Size 100ug
Source / Host bacteria
Species Reactivity human
Storage Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Substrate / Buffer In 20mM Tris pH7.5
UniProt Number P62258
Product Page Updated 2019-04-15T12:57:56.440Z

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