Article No
PAT-771B4Y-250
Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein
Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein
Proteins & Peptides
Specifications
| MW | 24.8kDa(220aa) |
| Accession Number | QHD43416.1 |
| Application | Electrophoresis |
| Article No | PAT-771B4Y-250 |
| Biosite Brand | Biosite Proteins |
| Country Availability | all |
| Concentration | 0.5 mg/ml |
| Description | Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD protein |
| Supplier | Nordic Biosite |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Entrez Gene ID | 43740568 |
| Gene Symbol | S |
| Notes | An epidemic of acute respiratory syndrome in humans, which appeared in Wuhan, China in December 2019, was caused by a novel coronavirus (SARS-CoV-2). This disease was named as "Coronavirus Disease 2019" (COVID-19). This virus shares highly homological sequence with SARS-CoV, and causes acute, highly lethal pneumonia coronavirus disease 2019 (COVID-19) with clinical symptoms similar to those reported for SARS-CoV and MERS-CoV. The genome of this and other emerging pathogenic human CoVs encodes four major structural proteins [spike (S), envelope (E), membrane (M), and nucleocapsid (N)], approximately 16 nonstructural proteins (nsp1-16), and five to eight accessory proteins. Among them, the S protein plays an essential role in viral attachment, fusion, entry, and transmission. It comprises an N-terminal S1 subunit responsible for virus-receptor binding and a C-terminal S2 subunit responsible for virus-cell membrane fusion. S1 is further divided into an N-terminal domain (NTD) and a receptor-binding domain (RBD). SARS-CoV-2 and SARS-CoV bind angiotensin-converting enzyme 2 (ACE2) while MERS-CoV binds dipeptidyl peptidase 4 (DPP4), as receptors on the host cell expressing ACE2 (e.g., pneumocytes, enterocytes) or DPP4 (e.g., liver or lung cells including Huh-7, MRC-5, and Calu-3). During infection, CoV first binds the host cell through interaction between its S1-RBD and the cell membrane receptor, triggering conformational changes in the S2 subunit that result in virus fusion and entry into the target cell. Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Previous Article No | PAT-771B4Y-250, 771B4Y-250 |
| Product Type | Proteins & Peptides |
| Purity | >95% |
| Sequence | RVQPTES IVRFPNITNL CPFGEVFNAT RFASVYAWNR KRISNCVADY SVLYNSASFS TFKCYGVSPT KLNDLCFTNV YADSFVIRGD EVRQIAPGQT GKIADYNYKL PDDFTGCVIA WNSNNLDSKV GGNYNYLYRL FRKSNLKPFE RDISTEIYQA GSTPCNGVEG FNCYFPLQSY GFQPTNGVGY QPYRVVVLSF ELLHAPATVC GPKK |
| Size | 250ug |
| Species Reactivity | virus |
| Storage | 4°C, -80°C |
| UniProt Number | P0DTC2 |
| Product Page Updated | 2024-04-17T08:28:29.021Z |