Proteins & Peptides

14-3-3 epsilon

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YWHAE, also known as 14-3-3 protein epsilon, is adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human YWHAE, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Article No

PAT-5FB1E9

Application

Electrophoresis

Species Reactivity

human

Size

100ug

Source / Host

bacteria

Specifications

MW 31.3 kDa (275aa) Confirmed by MALDI-TOF
NCBI Number NP_006752
Application Electrophoresis
Article No PAT-5FB1E9
BioSite Brand BioSite
Concentration 1 mg/ml (determined by Bradford assay)
Description YWHAE, also known as 14-3-3 protein epsilon, is adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human YWHAE, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Supplier BioSite
Domain 1-255aa
Keywords Signal Transduction,Apoptosis
Notes YWHAE, also known as 14-3-3 protein epsilon, is adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human YWHAE, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Alias Names 14-3-3 protein epsilon, 14-3-3E, HEL2, KCIP-1, MDCR, MDS
Product Type Proteins & Peptides
Purity > 90% by SDS-PAGE
Research Area Cell Signaling, Cell Death
Sequence MGSSHHHHHH SSGLVPRGSH MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV EDENQ
Size 100ug
Source / Host bacteria
Species Reactivity human
Storage Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Substrate / Buffer Liquid. In Phosphate Buffered Saline (pH7.4) containing 1mM DTT, 10% glycerol
UniProt Number P62258
Product Page Updated 2019-04-15T12:57:56.440Z

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