Article No
PAT-24M9TP-100
Recombinant SARS-CoV Spike RBD protein
Recombinant SARS-CoV Spike RBD protein
Proteins & Peptides
Specifications
| MW | 24.8kDa (220aa) |
| Accession Number | NP_828851.1 |
| Application | Electrophoresis |
| Article No | PAT-24M9TP-100 |
| Biosite Brand | Biosite Proteins |
| Country Availability | all |
| Concentration | 0.5 mg/ml |
| Description | Recombinant SARS-CoV Spike RBD protein |
| Supplier | Nordic Biosite |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Notes | Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle Eastern Respiratory Syndrome Coronavirus (MERS-CoV), and the recently identified novel Coronavirus (SARS-CoV-2) belong to the Coronaviridae family, genus Betacoronavirus, that has been related to important epidemiological outbreaks. SARS-CoV emerged in 2003 as a significant threat to human health. SARS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. It attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. A metallopeptidase, angiotensin-converting enzyme 2 (ACE-2), has been identified as a functional receptor for SARS-CoV through interaction with a receptor binding domain (RBD) located at the C-terminus of S1 subunit. Recombinant SARS-CoV spike RBD protein fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques. |
| Previous Article No | PAT-24M9TP-100, 24M9TP-100 |
| Product Type | Proteins & Peptides |
| Purity | >95% |
| Sequence | RVVPSG DVVRFPNITN LCPFGEVFNA TKFPSVYAWE RKKISNCVAD YSVLYNSTFF STFKCYGVSA TKLNDLCFSN VYADSFVVKG DDVRQIAPGQ TGVIADYNYK LPDDFMGCVL AWNTRNIDAT STGNYNYKYR YLRHGKLRPF ERDISNVPFS PDGKPCTPPA LNCYWPLNDY GFYTTTGIGY QPYRVVVLSF ELLNAPATVC GPKL |
| Size | 100ug |
| Species Reactivity | virus |
| Storage | 4°C, -80°C |
| UniProt Number | P59594 |
| Product Page Updated | 2024-04-17T08:28:29.021Z |