Article No
OKEH02577-96W
2, 3-Bisphosphoglycerate ELISA Kit
2, 3-Bisphosphoglycerate ELISA Kit
Assay & Detection
Specifications
| Application | ELISA |
| Article No | OKEH02577-96W |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Description | 2, 3-Bisphosphoglycerate ELISA Kit |
| Detection Method | Colorimetric, OD450 nm |
| Supplier | Aviva Systems Biology |
| Incubation Time | ~ 3 Hours |
| Notes | 2,3-Bisphosphoglyceric acid (2,3-Bisphosphoglycerate or 2,3-BPG, also known as 2,3-diphosphoglycerate or 2,3-DPG) is a three-carbon isomer of the glycolytic intermediate 1,3-bisphosphoglyceric acid (1,3-BPG). 2,3-BPG is present in human red blood cells (RBC; erythrocyte) at approximately 5 mmol/L. It binds with greater affinity to deoxygenated hemoglobin (e.g. when the red cell is near respiring tissue) than it does to oxygenated hemoglobin (e.g., in the lungs) due to spatial changes: 2,3-BPG (whose size is estimated at about 9 angstroms) fits in the deoxygenated hemoglobin configuration (11 angstroms), but not as well in the oxygenated (5 angstroms). It interacts with deoxygenated hemoglobin beta subunits by decreasing their affinity for oxygen, so it allosterically promotes the release of the remaining oxygen molecules bound to the hemoglobin, thus enhancing the ability of RBCs to release oxygen near tissues that need it most. 2,3-BPG is thus an allosteric effector. |
| Previous Article No | OKEH02577-96W-96 |
| Predicted Species Reactivity | multi species |
| Product Type | Assay & Detection |
| Range | 31.2-2000nmol/mL |
| Sensitivity | 9.7nmol/mL |
| Shipping Information | BLUE ICE |
| Size | 96 Wells |
| Species Reactivity | multi species |
| Product Page Updated | 2024-03-06T14:34:21.852Z |
