Article No
OCPB00172-0.1
Lysates
293 Lysate
HEK293 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HEK293 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Documentation
Suggested protocols
Specifications
| Additional Information | This product may be available in more sizes. Please inquire for size options. |
| Application | WB |
| Article No | OCPB00172-0.1 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL, RU |
| Concentration | 2 mg/ml |
| Description | HEK293 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HEK293 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
| Supplier | Aviva Systems Biology |
| Format | <b>Liquid</b> SDS sample buffer containing 50mM DTT |
| Keywords | Cell Lysate; |
| Notes | Transformed primary embryonal kidney |
| Product Type | Lysates |
| Shipping Information | BLUE ICE |
| Size | 0.1mg |
| Storage | -20°C |
| Product Page Updated | 2022-03-15T08:18:46.718Z |
Shipping info
The delivery time for this item is approximately 8-16 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.