Lysates

293 Lysate

HEK293 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HEK293 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Article No

OCPB00172-0.1

Application

WB

Size

0.1mg

Shipping Information

BLUE ICE

Documentation

Suggested protocols

Specifications

Additional Information This product may be available in more sizes. Please inquire for size options.
Application WB
Article No OCPB00172-0.1
Country Availability SE, FI, DK, NO, IS, EE, LV, LT, FO, GL, RU
Concentration 2 mg/ml
Description HEK293 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HEK293 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.
Supplier Aviva Systems Biology
Format <b>Liquid</b> SDS sample buffer containing 50mM DTT
Keywords Cell Lysate;
Notes Transformed primary embryonal kidney
Product Type Lysates
Shipping Information BLUE ICE
Size 0.1mg
Storage -20°C
Product Page Updated 2022-03-15T08:18:46.718Z

Shipping info

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