Article No
OASB01196-500UG
Specifications
| Accession Number | NM_000572.2, NP_000563.1 |
| Application | ELIspot, FC, IHC, WB |
| Article No | OASB01196-500UG |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | JES3-9D7 |
| Clone Type | monoclonal |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Description | Human IL-10 Antibody |
| Supplier | Aviva Systems Biology |
| Entrez Gene ID | 102133450,105576723,3586,694931 |
| Gene Symbol | IL10 |
| Immunogen | COS-expressed human IL-10 |
| Isotype | IgG1 |
| Notes | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. |
| Previous Article No | OASB01196-500UG-0.5 |
| Predicted Species Reactivity | monkey, virus |
| Product Type | Antibodies Primary |
| Protocol | ELISA Capture: Purified JES3-9D7 monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human and/or viral IL-10 proteins.1-5 Purified JES3-9D7 antibody should be paired with biotinylated JES3-12G8 antibody (OASB01199) as the detection antibody, with purified recombinant human IL-10 as the standard. Immunofluorescence/Flow Cytometry: The JES3-9D7 antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant human IL-10 or unlabeled Rat IgG1 should be used as a specificity control. Western Blotting: The purified JES3-9D7 antibody is also useful for Western blotting applications. A concentration of 1-5ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig, OASB01897) has been found to enable detection of <= 100 ng/lane of recombinant human IL-10 under reducing conditions. Neutralization: The LE/AF form of JES3-9D7 can be used for neutralizing human/viral IL-10 bioactivity. A suitable LE/AF Rat IgG1 (OASB00095) should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. |
| Shipping Information | BLUE ICE |
| Size | 0.5 mg |
| Source / Host | rat |
| Species Reactivity | human |
| Storage | 4°C |
| UniProt Number | G7NVB0, P22301, P51496 |
| Product Page Updated | 2024-03-06T14:34:21.852Z |
