Antibodies Primary

ER-ALPHA

NEW
ER-ALPHA Antibody (OAAD00234)

Article No

OAAD00234

Application

WB, IHC, ELISA

Species Reactivity

human

Size

0.1mg

Clone

2B7

Source / Host

mouse

Shipping Information

Wet Ice

Product insert

Suggested protocols

Specifications

Additional Information The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.
Application WB, IHC, ELISA
Article No OAAD00234
Clone 2B7
Clone Type monoclonal
Description ER-ALPHA Antibody (OAAD00234)
Supplier Aviva Systems Biology
Immunogen Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. Coli.
Isotype IgG1
Keywords Monoclonal;
Notes The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.
Alias Names ER, ESR1, Era, ESRA
Product Type Antibodies Primary
Protocol <b>Western Bloting: </b> 1/500 - 1/2000.<br/><b>Immunohistochemistry: </b> 1/200 - 1/1000.<br/><b>ELISA: </b>Propose dilution 1/10000.<br/>Not yet tested in other applications.<br/>Determining optimal working dilutions by titration test.
Shipping Information Wet Ice
Size 0.1mg
Source / Host mouse
Species Reactivity human
Storage Store at 4C, for long term storage, store at -20C.
Substrate / Buffer Purified antibody in PBS containing 0.03% sodium azide.
Product Page Updated 2019-01-11T14:50:38.678Z

Shipping info

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