Article No
M054-3
Anti-Caspase-9 (Human) mAb
Anti-Caspase-9 (Human) mAb
Antibodies Primary
Specifications
| Application | WB |
| Article No | M054-3 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | 5B4 |
| Clone Type | monoclonal |
| Concentration | 1 mg/mL |
| Conjugation | Unconjugated |
| Description | Anti-Caspase-9 (Human) mAb |
| Supplier | MBL |
| Immunogen | Recombinant Caspase-9 (:1-250 aa terminal N) |
| Isotype | IgG1 |
| Notes | Apoptosis is a major form of cell death characterized by morphological features including chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signaling pathways that lead to the recruitment and activation of caspases, a family of cysteine-containing, aspartate-specific proteases. Caspases exist as inactive proenzymes in cells and are activated through their processing into two subunits in response to apoptotic stimulation. Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitment to cell death. Caspase-7 (also known as Mch-3/ ICE-LAP3 / CMH-1) is a 35 kDa protein that has the highest similarity to caspase-3 (52% amino acid identity) among all caspase members. It has been identified as one of the ''effector'' caspases (which include caspase 3, 6, 7) that are cleaved by ''initiator'' caspases (which include caspase 8, 9) into active form, and then, in turn, cleave various cellular proteins for apoptosis. Recent study says that in Fas-mediated hepatocyte apoptosis, active caspase-7 is associated almost exclusively with the mitochondrial and microsomal fractions, whereas active caspase-3 is confined primarily to the cytosol. It implies a different role of caspase-3 and -7 in the execution of apoptosis. |
| Product Type | Antibodies Primary |
| Research area | Cell Death |
| Shipping Information | 4°C |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human, mouse, rat |
| Stability | 1 year |
| Storage | -20°C |
| Substrate / Buffer | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. No preservative iscontained. |
| Technical Specifications | Apoptosis is a major form of cell death characterized by morphological features including chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. These morphological changes occur via signaling pathways that lead to the recruitment and activation of caspases, a family of cysteine-containing, aspartate-specific proteases. Caspases exist as inactive proenzymes in cells and are activated through their processing into two subunits in response to apoptotic stimulation. Activated caspases cleave a variety of important cellular proteins, other caspases, and Bcl-2 family members, leading to a commitment to cell death. Caspase-7 (also known as Mch-3/ ICE-LAP3 / CMH-1) is a 35 kDa protein that has the highest similarity to caspase-3 (52% amino acid identity) among all caspase members. It has been identified as one of the ''effector'' caspases (which include caspase 3, 6, 7) that are cleaved by ''initiator'' caspases (which include caspase 8, 9) into active form, and then, in turn, cleave various cellular proteins for apoptosis. Recent study says that in Fas-mediated hepatocyte apoptosis, active caspase-7 is associated almost exclusively with the mitochondrial and microsomal fractions, whereas active caspase-3 is confined primarily to the cytosol. It implies a different role of caspase-3 and -7 in the execution of apoptosis. |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
