Article No
M041-3
Anti-PML (Human) mAb
Anti-PML (Human) mAb
Antibodies Primary
Specifications
| Application | FC, ICC, IP, WB |
| Article No | M041-3 |
| Country Availability | SE, FI, DK, NO, IS, EE, LV, LT, FO, GL |
| Clone | 1B9 |
| Clone Type | monoclonal |
| Concentration | 1 mg/mL |
| Conjugation | Unconjugated |
| Description | Anti-PML (Human) mAb |
| Supplier | MBL |
| Immunogen | Full-length recombinant human PML |
| Isotype | IgG1 |
| Notes | Acute promyelocytic leukemia (APL) is associated with a t(15;17) translocation that creates the promyelocyte-retinoic acid receptor α (PML-RARα) fusion protein and successfully differentiated by all-trans-retinoic acid (ATRA). PML-RARα consists of all amino acid of RARα except the first 59 amino acids and includes its DNA-binding and ligand-binding domains. PML-RARα contains the functional domains of PML which includes the DNA binding and dimerization property. Thus, the functions of PML and/or retinoid X receptor are sequestrated by PML-RARα in a dominant negative manner. In APL cells, the PML-RARα and PML are immunologically localized as microgranules in the nuclei and cytoplasm, whereas in normal cells, PML is immunologically found as a discrete speckled pattern in nuclei. The ATRA treatment of the APL cells triggers a reorganization of PML to generate normal localization. Anti-PML antibody is a strong tool for the detection of the chromosomal translocation t(15;17) on the APL cells and/or determination of the sensitivity of the APL cells to the ATRA differentiation of hematopoietic cells and apoptosis. |
| Product Type | Antibodies Primary |
| Shipping Information | 4°C |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Stability | 1 year |
| Storage | 4°C |
| Substrate / Buffer | 100 μg IgG in 100 μl volume of PBS containing 50% glycerol, pH 7.2. Contains no preservative. |
| Technical Specifications | Acute promyelocytic leukemia (APL) is associated with a t(15;17) translocation that creates the promyelocyte-retinoic acid receptor α (PML-RARα) fusion protein and successfully differentiated by all-trans-retinoic acid (ATRA). PML-RARα consists of all amino acid of RARα except the first 59 amino acids and includes its DNA-binding and ligand-binding domains. PML-RARα contains the functional domains of PML which includes the DNA binding and dimerization property. Thus, the functions of PML and/or retinoid X receptor are sequestrated by PML-RARα in a dominant negative manner. In APL cells, the PML-RARα and PML are immunologically localized as microgranules in the nuclei and cytoplasm, whereas in normal cells, PML is immunologically found as a discrete speckled pattern in nuclei. The ATRA treatment of the APL cells triggers a reorganization of PML to generate normal localization. Anti-PML antibody is a strong tool for the detection of the chromosomal translocation t(15;17) on the APL cells and/or determination of the sensitivity of the APL cells to the ATRA differentiation of hematopoietic cells and apoptosis. |
| Product Page Updated | 2023-12-29T16:20:16.638Z |
