Article No
LS-C751509-20
Application | IHC, ISH |
Article No | LS-C751509-20 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | BTN/2032R |
Clone Type | monoclonal |
Concentration | 200 ug/ml |
Conjugation | Unconjugated |
Description | Biotin antibody LS-C751509 is an unconjugated rabbit recombinant monoclonal antibody to all species Biotin. Validated for IHC and ISH. |
Recommended Dilution | IHC-P (0.5 - 1 ug/ml) |
Supplier | LifeSpan Biosciences |
Immunogen | Biotinylated sheep immunoglobulin. Antigen Molecular Weight: 244Da |
Isotype | IgG |
Notes | Biotin antibody LS-C751509 is an unconjugated rabbit recombinant monoclonal antibody to all species Biotin. Validated for IHC and ISH. |
Product Type | Antibodies Primary |
Protocol | Flow Cytometry (0.5-1 ug/million cells) - Immunofluorescence (0.5-1 ug/ml) - In situ Nucleic Acid Hybridization; Western Blotting; Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.) - Optimal dilution for a specific application should be determined. |
Purification | Protein A Purified |
Shipping Information | RT |
Size | 20 µg |
Source / Host | rabbit |
Species Reactivity | multi species |
Storage | 4°C |
Substrate / Buffer | 10 mM PBS, 0.05% BSA, 0.05% Sodium Azide |
Technical Specifications | It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. |
Product Page Updated | 2024-03-11T13:31:19.399Z |