Article No
LS-C751358-20
NCBI Number | 6490 |
Application | IHC |
Article No | LS-C751358-20 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | PMEL/1825R |
Clone Type | monoclonal |
Concentration | 200 ug/ml |
Conjugation | Unconjugated |
Description | Gp100 antibody LS-C751358 is an unconjugated rabbit recombinant monoclonal antibody to human gp100 (PMEL / SILV). Validated for IHC. |
Recommended Dilution | IHC-P (0.5 - 1 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 6490 |
Gene Symbol | PMEL |
Immunogen | Recombinant full-length human gp100 protein. Antigen Molecular Weight: 90-100 kDa |
Isotype | IgG |
Notes | Gp100 antibody LS-C751358 is an unconjugated rabbit recombinant monoclonal antibody to human gp100 (PMEL / SILV). Validated for IHC. |
Alias Names | PMEL, gp100, Melanocyte protein PMEL, Me20m/me20s, p100, PMEL17, Premelanosome protein, SIL, Silver homolog (mouse), Silver, mouse, homolog of, Silver locus protein homolog, ME20-M, Me20-m/me20-s, ME20M, Melanocyte protein mel 17, Melanocyte protein Pmel 17, Silver (mouse homolog) like, D12S53E, ME20, Melanosomal matrix protein17, SILV |
Product Type | Antibodies Primary |
Protocol | Immunohistology (Formalin-fixed - 0.5-1.0 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined. |
Purification | Protein A/G Purified |
Shipping Information | RT |
Size | 20 µg |
Source / Host | rabbit |
Species Reactivity | human |
Storage | 4°C |
Substrate / Buffer | 10 mM PBS, 0.05% BSA, 0.05% Sodium Azide |
Technical Specifications | By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa). |
Product Page Updated | 2024-03-11T13:31:19.399Z |