Article No
LS-C63359-100
Application | ELISA, IP, WB |
Article No | LS-C63359-100 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone Type | monoclonal |
Conjugation | Unconjugated |
Description | 14-3-3 Binding Motif antibody LS-C63359 is an unconjugated mouse monoclonal antibody to 14-3-3 Binding Motif (pSer). Validated for ELISA, IP and WB. |
Recommended Dilution | ELISA (1:1000), IP (1:20), WB (1:4000) |
Supplier | LifeSpan Biosciences |
Immunogen | Synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled). |
Isotype | IgG1 |
Notes | 14-3-3 Binding Motif antibody LS-C63359 is an unconjugated mouse monoclonal antibody to 14-3-3 Binding Motif (pSer). Validated for ELISA, IP and WB. |
Product Type | Antibodies Primary |
Protocol | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:4000. Immunoprecipitation: 1:20 (Denatured proteins). ELISA: 1:1000. |
Purification | Ascites |
Shipping Information | RT |
Size | 100 µl |
Source / Host | mouse |
Substrate / Buffer | 10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol |
Technical Specifications | Binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA, recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. Weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding non-phosphorylated sequences. |
Product Page Updated | 2024-04-12T09:51:03.289Z |
Volume | 100 µl |