Article No
LS-C60054-1
GFP
GFP antibody LS-C60054 is a TRITC-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for FLISA, IF and WB.
Antibodies Primary
Specifications
| Application | FLISA, IF, WB |
| Article No | LS-C60054-1 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone Type | polyclonal |
| Concentration | 1 mg/ml |
| Conjugation | TRITC |
| Description | GFP antibody LS-C60054 is a TRITC-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for FLISA, IF and WB. |
| Recommended Dilution | FLISA (1:20000), IF (1:500 - 1:2500), WB (1:10000) |
| Supplier | LifeSpan Biosciences |
| Immunogen | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Isotype | IgG |
| Notes | GFP antibody LS-C60054 is a TRITC-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for FLISA, IF and WB. |
| Alias Names | GFP |
| Product Type | Antibodies Primary |
| Protocol | Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISA for direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited to titrate GFP in solution using either form of the antibody as the capture or detection antibodies. The detection antibody is typically conjugated to biotin and complexed with streptavidin-HRP. Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Peroxidase conjugated anti-GFP assayed by immunoblot shows a 42 kD band when reacted with GFP on a western blot. Optimal titers for applications should be determined by the researcher. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
| Purification | Affinity Purified |
| Shipping Information | RT |
| Size | 1 mg |
| Source / Host | goat |
| Species Reactivity | invertebrates |
| Storage | -20°C |
| Substrate / Buffer | 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide. |
| Technical Specifications | anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins |
| Product Page Updated | 2024-04-12T09:51:03.289Z |
