Article No
LS-C391600-100
CHGA / Chromogranin A
Chromogranin A antibody LS-C391600 is an unconjugated mouse monoclonal antibody to Chromogranin A (CHGA) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF and IHC.
Antibodies Primary
Specifications
| NCBI Number | 1113 |
| Application | FC, IF, IHC |
| Article No | LS-C391600-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | LK2H10 + PHE5 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Chromogranin A antibody LS-C391600 is an unconjugated mouse monoclonal antibody to Chromogranin A (CHGA) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF and IHC. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.25 - 0.5 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 1113 |
| Gene Symbol | CHGA |
| Immunogen | Human pheochromocytoma cells were used as the immunogen for this Chromogranin A antibody cocktail (LK2H10 & PHE5). |
| Isotype | IgG1 |
| Notes | Chromogranin A antibody LS-C391600 is an unconjugated mouse monoclonal antibody to Chromogranin A (CHGA) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF and IHC. |
| Alias Names | CHGA, Chromogranin A, Chromogranin-A, Pancreastatin, Pituitary secretory protein I, Vasostatin I, SP-I, Vasostatin II |
| Product Type | Antibodies Primary |
| Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin. The optimal dilution of the Chromogranin A antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human, monkey, mouse, pig/porcine, rat |
| Storage | 4°C |
| Substrate / Buffer | Sterile Solution PBS |
| Technical Specifications | Human CHGA / Chromogranin A |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
