Article No
LS-C391547-7
NCBI Number | 598 |
Application | FC, IF, IHC, WB |
Article No | LS-C391547-7 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | 2H12 |
Clone Type | monoclonal |
Conjugation | Unconjugated |
Description | BCL-XL antibody LS-C391547 is an unconjugated mouse monoclonal antibody to BCL-XL (BCL2L1) (aa3-14) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB. |
Recommended Dilution | IHC-P (1:1) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 598 |
Gene Symbol | BCL2L1 |
Immunogen | Synthetic peptide (QSNRELVVDFLS, aa3-14 of UniProt Q07817) from the human protein. Percent identity by BLAST analysis: Human, Mouse, Rat, Ferret, Sheep, Cat, Bovine, Bat, Hamster, Elephant, Rabbit, Horse, Pig, Dog (100%); Guinea pig (92%); Opossum, Lizard (83%). |
Isotype | IgG2a |
Notes | BCL-XL antibody LS-C391547 is an unconjugated mouse monoclonal antibody to BCL-XL (BCL2L1) (aa3-14) from human. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB. |
Alias Names | BCL2L1, Apoptosis regulator Bcl-X, Bcl-xL, BCL-XL/S, BCLXS, Bcl-2-like protein 1, BCL2-like 1, BCL2L, Bcl-X, Bcl-xS, BCLXL, PPP1R52, Bcl2-L-1, BCLX |
Product Type | Antibodies Primary |
Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 27kDa, identified as the Bcl-X protein. This mAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins. The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Purification | Protein G Purified |
Shipping Information | RT |
Size | 7 ml |
Source / Host | mouse |
Species Reactivity | cow/bovine, dog/canine, hamster, horse/equine, human, mammals, mouse, pig/porcine, rabbit, rat, sheep/ovine |
Storage | 4°C |
Substrate / Buffer | PBS, 0.01% BSA, 0.05% sodium azide |
Technical Specifications | Human BCL2L1 / Bcl-xL |
Product Page Updated | 2024-04-12T09:20:06.597Z |
Volume | 7 ml |