Article No
LS-C391159-100
ZAP70
ZAP70 antibody LS-C391159 is an unconjugated mouse monoclonal antibody to human ZAP70. Validated for Flow, IF, IHC and WB.
Antibodies Primary
Specifications
| NCBI Number | 7535 |
| Application | FC, IF, IHC, WB |
| Article No | LS-C391159-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 2F3.2 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | ZAP70 antibody LS-C391159 is an unconjugated mouse monoclonal antibody to human ZAP70. Validated for Flow, IF, IHC and WB. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 7535 |
| Gene Symbol | ZAP70 |
| Immunogen | Recombinant human protein (including amino acids 1-254 and the SH2 domains). |
| Isotype | IgG2a k |
| Notes | ZAP70 antibody LS-C391159 is an unconjugated mouse monoclonal antibody to human ZAP70. Validated for Flow, IF, IHC and WB. |
| Alias Names | ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70 |
| Product Type | Antibodies Primary |
| Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis. Optimal dilution of the ZAP-70 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | Sterile Solution PBS |
| Technical Specifications | Human ZAP70 |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
