Article No
LS-C391067-100
NCBI Number | 7124 |
Application | ELISA, FC, IF, IHC |
Article No | LS-C391067-100 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | MAb1 |
Clone Type | monoclonal |
Concentration | 0.2 mg/ml |
Conjugation | Unconjugated |
Description | TNF Alpha antibody LS-C391067 is an unconjugated mouse monoclonal antibody to human TNF Alpha. Validated for ELISA, Flow, IF and IHC. |
Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (1 - 2 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 7124 |
Gene Symbol | TNF |
Immunogen | Recombinant human protein. |
Isotype | IgM k |
Notes | TNF Alpha antibody LS-C391067 is an unconjugated mouse monoclonal antibody to human TNF Alpha. Validated for ELISA, Flow, IF and IHC. |
Alias Names | TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2 |
Product Type | Antibodies Primary |
Protocol | This mAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex. Optimal dilution of the TNFa antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Purification | PEG Precipitated |
Shipping Information | RT |
Size | 100 µg |
Source / Host | mouse |
Species Reactivity | human |
Storage | 4°C |
Substrate / Buffer | 0.01% BSA, 0.05% sodium azide |
Technical Specifications | Human TNF Alpha |
Product Page Updated | 2024-04-12T09:20:06.597Z |