Article No
LS-C390930-100
Application | FC, IF, IHC |
Article No | LS-C390930-100 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | MAC387 |
Clone Type | monoclonal |
Concentration | 0.2 mg/ml |
Conjugation | Unconjugated |
Description | MRP14 antibody LS-C390930 is an unconjugated mouse monoclonal antibody to MRP14 (S100A9) from human. It is reactive with human, mouse and rat. Validated for Flow, IF and IHC. |
Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.5 - 1 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 6280 |
Gene Symbol | S100A9 |
Immunogen | Aaffinity purified monocyte membrane preparation. |
Isotype | IgG1 k |
Notes | MRP14 antibody LS-C390930 is an unconjugated mouse monoclonal antibody to MRP14 (S100A9) from human. It is reactive with human, mouse and rat. Validated for Flow, IF and IHC. |
Alias Names | , Mature Macrophage Marker |
Product Type | Antibodies Primary |
Protocol | Recognizes S100A9/MRP14 and the MRP8/14 complex by binding to the MRP14 portion, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. Optimal dilution of the MRP8 + MPR14 antibody should be determined by the researcher. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Purification | Protein G Purified |
Shipping Information | RT |
Size | 100 µg |
Source / Host | mouse |
Species Reactivity | human, mouse, rat |
Storage | 4°C |
Substrate / Buffer | PBS, pH 7.4, 0.1 mg/ml BSA, 0.05% Sodium Azide |
Technical Specifications | Human Macrophage |
Product Page Updated | 2024-04-12T09:20:06.597Z |