Article No
LS-C390859-7
PODXL / Podocalyxin
Podocalyxin antibody LS-C390859 is an unconjugated mouse monoclonal antibody to Podocalyxin (PODXL) (Extracellular Domain) from human. It is reactive with human, rat and rabbit. Validated for Flow, IF and IHC.
Antibodies Primary
Specifications
| NCBI Number | 5420 |
| Application | FC, IF, IHC |
| Article No | LS-C390859-7 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 2A4 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | Podocalyxin antibody LS-C390859 is an unconjugated mouse monoclonal antibody to Podocalyxin (PODXL) (Extracellular Domain) from human. It is reactive with human, rat and rabbit. Validated for Flow, IF and IHC. |
| Recommended Dilution | IHC-P (1:1) |
| Supplier | LifeSpan Biosciences |
| Domain | Extracellular Domain |
| Entrez Gene ID | 5420 |
| Gene Symbol | PODXL |
| Immunogen | Recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human PODXL. |
| Isotype | IgM |
| Notes | Podocalyxin antibody LS-C390859 is an unconjugated mouse monoclonal antibody to Podocalyxin (PODXL) (Extracellular Domain) from human. It is reactive with human, rat and rabbit. Validated for Flow, IF and IHC. |
| Alias Names | PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81 |
| Product Type | Antibodies Primary |
| Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Podocalyxin/PODXL is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Optimal dilution of the Podocalyxin antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Purification | PEG Precipitated |
| Shipping Information | RT |
| Size | 7 ml |
| Source / Host | mouse |
| Species Reactivity | human, rabbit, rat |
| Storage | 4°C |
| Substrate / Buffer | PBS, 0.01% BSA, 0.05% sodium azide |
| Technical Specifications | Human PODXL / Podocalyxin |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
| Volume | 7 ml |
