Article No
LS-C390699-100
EMA / MUC1
MUC1 antibody LS-C390699 is an unconjugated mouse monoclonal antibody to MUC1 (EMA) from human. It is reactive with human and mouse. Validated for ELISA, Flow, IF and IHC.
Antibodies Primary
Specifications
| NCBI Number | 4582 |
| Application | ELISA, FC, IF, IHC |
| Article No | LS-C390699-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 139H2 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | MUC1 antibody LS-C390699 is an unconjugated mouse monoclonal antibody to MUC1 (EMA) from human. It is reactive with human and mouse. Validated for ELISA, Flow, IF and IHC. |
| Recommended Dilution | FC (0.25 - 0.5 ug/10e6 cells), IF (1 - 2 ug/ml), IHC-P (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 4582 |
| Gene Symbol | MUC1 |
| Immunogen | Human milk-fat globule membranes (HMFGM) |
| Isotype | IgG1 k |
| Notes | MUC1 antibody LS-C390699 is an unconjugated mouse monoclonal antibody to MUC1 (EMA) from human. It is reactive with human and mouse. Validated for ELISA, Flow, IF and IHC. |
| Alias Names | MUC1, CA15-3, CD227, Carcinoma-associated mucin, CD227 antigen, DF3 antigen, Episialin, Epithelial membrane antigen, H23 antigen, H23AG, Krebs von den Lungen-6, MAM6, MUC1/ZD, MUC-1/SEC, Mucin-1, Pem, KL-6, MUC-1/X, Tumor-associated mucin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, PUM, EMA, MUC-1, Mucin 1, transmembrane |
| Product Type | Antibodies Primary |
| Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA. The dominant epitope of this mAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Optimal dilution of the MUC1 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human, mouse |
| Storage | 4°C |
| Substrate / Buffer | Sterile Solution PBS |
| Technical Specifications | Human EMA / MUC1 |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
