Article No
LS-C390650-7
MITF
MITF antibody LS-C390650 is an unconjugated mouse monoclonal antibody to human MITF (N-Terminus). Validated for Flow, IF and IHC.
Antibodies Primary
Specifications
| NCBI Number | 4286 |
| Application | FC, IF, IHC |
| Article No | LS-C390650-7 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | d5 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | MITF antibody LS-C390650 is an unconjugated mouse monoclonal antibody to human MITF (N-Terminus). Validated for Flow, IF and IHC. |
| Recommended Dilution | IHC-P (1:1) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 4286 |
| Gene Symbol | MITF |
| Immunogen | An N-Terminus fragment of human Mi protein. |
| Isotype | IgG1 k |
| Notes | MITF antibody LS-C390650 is an unconjugated mouse monoclonal antibody to human MITF (N-Terminus). Validated for Flow, IF and IHC. |
| Alias Names | MITF, BHLHe32, CMM8, MI, Waardenburg syndrome, type 2A, WS2, WS2A |
| Product Type | Antibodies Primary |
| Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi. Optimal dilution of the MITF antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 7 ml |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | PBS, 0.01% BSA, 0.05% sodium azide |
| Technical Specifications | Human MITF |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
| Volume | 7 ml |
