Article No
LS-C390625-20
MAP3K1 / MEKK1
MEKK1 antibody LS-C390625 is an unconjugated mouse monoclonal antibody to human MEKK1 (MAP3K1) (aa1211-1310). Validated for Flow, IF, IHC and WB.
Antibodies Primary
Specifications
| NCBI Number | 4214 |
| Application | FC, IF, IHC, WB |
| Article No | LS-C390625-20 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 2F6 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | MEKK1 antibody LS-C390625 is an unconjugated mouse monoclonal antibody to human MEKK1 (MAP3K1) (aa1211-1310). Validated for Flow, IF, IHC and WB. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (1 - 2 ug/ml), IHC-P (0.5 - 1 ug/ml), WB (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 4214 |
| Gene Symbol | MAP3K1 |
| Immunogen | Partial recombinant protein (aa1211-1310). |
| Isotype | IgG2a k |
| Notes | MEKK1 antibody LS-C390625 is an unconjugated mouse monoclonal antibody to human MEKK1 (MAP3K1) (aa1211-1310). Validated for Flow, IF, IHC and WB. |
| Alias Names | MAP3K1, MAPK/ERK kinase kinase 1, MAPKKK1, MAP/ERK kinase kinase 1, MEK kinase 1, MEKK, MEKK 1, MEKK1, SRXY6 |
| Product Type | Antibodies Primary |
| Protocol | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli, including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (MEK kinase- 5). MEK kinase-1 activates the ERK and c-Jun NH2-terminal kinase (JNK) pathways by phosphorylation of MAP2K1 and MAP2K4, and also activates the central protein kinases of the NF-kB pathway, CHUK and IKBKB. Additionally, MEK kinase-1 uses an E3 ligase through its PHD domain, a RING-finger-like structure, to target proteins for degradation through ubiquitination. Optimal dilution of the MAP3K1 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 20 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | 1X PBS, 0.1mg/ml BSA, 0.05% sodium azide. |
| Technical Specifications | Human MAP3K1 / MEKK1 |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
