Article No
LS-C390566-100
LAMC1 / Laminin Gamma 1
Laminin Gamma 1 antibody LS-C390566 is an unconjugated rat monoclonal antibody to Laminin Gamma 1 (LAMC1) from mouse. It is reactive with human and mouse. Validated for Flow, IF and IHC.
Antibodies Primary
Specifications
| NCBI Number | 3915 |
| Application | FC, IF, IHC |
| Article No | LS-C390566-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | A5 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | Laminin Gamma 1 antibody LS-C390566 is an unconjugated rat monoclonal antibody to Laminin Gamma 1 (LAMC1) from mouse. It is reactive with human and mouse. Validated for Flow, IF and IHC. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (1 - 2 ug/ml), IHC-P (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 3915 |
| Gene Symbol | LAMC1 |
| Immunogen | Murine EHS laminin preparation. |
| Isotype | IgG2a k |
| Notes | Laminin Gamma 1 antibody LS-C390566 is an unconjugated rat monoclonal antibody to Laminin Gamma 1 (LAMC1) from mouse. It is reactive with human and mouse. Validated for Flow, IF and IHC. |
| Alias Names | LAMC1, Laminin subunit gamma-1, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-1 subunit gamma, Laminin-4 subunit gamma, Laminin-9 subunit gamma, Laminin B2 chain, Laminin gamma-1 chain, Laminin-3 subunit gamma, Laminin-6 subunit gamma, S-laminin subunit gamma, Laminin Gamma 1, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, S-LAM gamma |
| Product Type | Antibodies Primary |
| Protocol | Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This mAb reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific mAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This mAb stains uniformly all human and murine basement membranes. Optimal dilution of the Laminin gamma 1 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | rat |
| Species Reactivity | human, mouse |
| Storage | 4°C |
| Substrate / Buffer | 0.01% BSA, 0.05% sodium azide |
| Technical Specifications | Mouse LAMC1 / Laminin Gamma 1 |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
