Article No
LS-C390374-20
ICAM3 / CD50
CD50 antibody LS-C390374 is an unconjugated mouse monoclonal antibody to human CD50 (ICAM3). Validated for Flow, IF and IHC.
Antibodies Primary
Specifications
| NCBI Number | 3385 |
| Application | FC, IF, IHC |
| Article No | LS-C390374-20 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 101-1D2 |
| Clone Type | monoclonal |
| Concentration | 0.2 mg/ml |
| Conjugation | Unconjugated |
| Description | CD50 antibody LS-C390374 is an unconjugated mouse monoclonal antibody to human CD50 (ICAM3). Validated for Flow, IF and IHC. |
| Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.5 - 1 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 3385 |
| Gene Symbol | ICAM3 |
| Immunogen | Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody. |
| Isotype | IgG2a k |
| Notes | CD50 antibody LS-C390374 is an unconjugated mouse monoclonal antibody to human CD50 (ICAM3). Validated for Flow, IF and IHC. |
| Alias Names | ICAM3, CD50, CDW50, ICAM-R, CD50 antigen, ICAM-3 |
| Product Type | Antibodies Primary |
| Protocol | Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues. Optimal dilution of the ICAM-3 antibody should be determined by the researcher. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 20 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | 0.01% BSA, 0.05% sodium azide |
| Technical Specifications | Human ICAM3 / CD50 |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
