Article No
LS-C389959-20
NCBI Number | 1026 |
Application | FC, IF, IHC |
Article No | LS-C389959-20 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | CIP1/823 + DCS-60.2 |
Clone Type | monoclonal |
Concentration | 0.2 mg/ml |
Conjugation | Unconjugated |
Description | P21 antibody LS-C389959 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for Flow, IF and IHC. |
Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (1 - 2 ug/ml), IHC-P (2 - 4 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 1026 |
Gene Symbol | CDKN1A |
Immunogen | Recombinant full-length human protein (CIP1/823 and DCS-60.2). |
Isotype | IgG |
Notes | P21 antibody LS-C389959 is an unconjugated mouse monoclonal antibody to human p21 (CDKN1A / WAF1). Validated for Flow, IF and IHC. |
Alias Names | CDKN1A, CDKN1, CIP1, CDK-interacting protein 1, DNA synthesis inhibitor, p21CIP1, MDA6, PIC1, SDI1, WAF1, CAP20, CDK-interaction protein 1, MDA-6, p21 |
Product Type | Antibodies Primary |
Protocol | This mAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This mAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). Optimal dilution of the p21 antibody to be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Purification | Protein G Purified |
Shipping Information | RT |
Size | 20 µg |
Source / Host | mouse |
Species Reactivity | human |
Storage | 4°C |
Substrate / Buffer | 0.01% BSA, 0.05% sodium azide |
Technical Specifications | Human CDKN1A / WAF1 / p21 |
Product Page Updated | 2024-04-12T09:20:06.597Z |