Article No
LS-C389950-100
NCBI Number | 1027 |
Application | FC, IF, IHC, WB |
Article No | LS-C389950-100 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | DCS-72.F6 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | P27 Kip1 antibody LS-C389950 is an unconjugated mouse monoclonal antibody to p27 Kip1 (CDKN1B) from mouse. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB. |
Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.25 - 0.5 ug/ml), WB (0.5 - 1 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 1027 |
Gene Symbol | CDKN1B |
Immunogen | Mouse recombinant protein. |
Isotype | IgG1 k |
Notes | P27 Kip1 antibody LS-C389950 is an unconjugated mouse monoclonal antibody to p27 Kip1 (CDKN1B) from mouse. It is reactive with human, mouse, rat and other species. Validated for Flow, IF, IHC and WB. |
Alias Names | CDKN1B, KIP1, MEN1B, P27 Kip1, p27KIP1, MEN4, CDKN4 |
Product Type | Antibodies Primary |
Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues. Optimal dilution of the p27 antibody to be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. |
Purification | Protein G Purified |
Shipping Information | RT |
Size | 100 µg |
Source / Host | mouse |
Species Reactivity | human, monkey, mouse, rat |
Storage | 4°C |
Substrate / Buffer | Sterile Solution PBS |
Technical Specifications | Mouse SSSCA1 / p27 |
Product Page Updated | 2024-03-11T11:55:05.043Z |