Article No
LS-C389673-100
Application | ELISA, FC, IF, IHC, IP, WB |
Article No | LS-C389673-100 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | UCHL-1 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | CD45RO antibody LS-C389673 is an unconjugated mouse monoclonal antibody to CD45RO from human. It is reactive with human, chimpanzee and marmoset. Validated for ELISA, Flow, IF, IHC, IP and WB. |
Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (1 - 2 ug/ml), IHC-P (0.5 - 1 ug/ml) |
Supplier | LifeSpan Biosciences |
Immunogen | Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as the immunogen. |
Isotype | IgG2a k |
Notes | CD45RO antibody LS-C389673 is an unconjugated mouse monoclonal antibody to CD45RO from human. It is reactive with human, chimpanzee and marmoset. Validated for ELISA, Flow, IF, IHC, IP and WB. |
Product Type | Antibodies Primary |
Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. ELISA: Order BSA-free format for coating. |
Purification | Protein G Purified |
Shipping Information | RT |
Size | 100 µg |
Source / Host | mouse |
Species Reactivity | human, mammals |
Storage | 4°C |
Substrate / Buffer | Sterile Solution PBS |
Technical Specifications | Human CD45RO |
Product Page Updated | 2024-04-12T09:20:06.597Z |