Article No
LS-C389436-100
NCBI Number | 768 |
Application | ELISA, FC, IF, IHC, IP, WB |
Article No | LS-C389436-100 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | 66.4.C2 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | Carbonic Anhydrase IX antibody LS-C389436 is an unconjugated mouse monoclonal antibody to Carbonic Anhydrase IX (CA9) from human. It is reactive with human and horse. Validated for ELISA, Flow, IF, IHC, IP and WB. |
Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (1 - 2 ug/ml), IHC-P (0.5 - 1 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 768 |
Gene Symbol | CA9 |
Immunogen | The microsomal fraction of human renal cortical tissue homogenate. |
Isotype | IgG2b k |
Notes | Carbonic Anhydrase IX antibody LS-C389436 is an unconjugated mouse monoclonal antibody to Carbonic Anhydrase IX (CA9) from human. It is reactive with human and horse. Validated for ELISA, Flow, IF, IHC, IP and WB. |
Alias Names | CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, Carbonic dehydratase, G250, MN, Membrane antigen MN, p54/58N, RCC-associated antigen G250, RCC-associated protein G250, Carbonic anhydrase 9, PMW1 |
Product Type | Antibodies Primary |
Protocol | The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This antibody recognizes a glycoprotein of ~200kDa, identified as renal cell carcinoma protein, carbonic anhydrase IX / CAIX, or gp200. Its epitope resides in the carbohydrate domain of the protein. The CAIX antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, it is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This CAIX antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation, especially those showing tubular differentiation. |
Purification | Protein G Purified |
Shipping Information | RT |
Size | 100 µg |
Source / Host | mouse |
Species Reactivity | horse/equine, human |
Storage | 4°C |
Substrate / Buffer | Sterile Solution PBS |
Technical Specifications | Human CA9 / Carbonic Anhydrase IX |
Product Page Updated | 2024-03-11T11:55:05.043Z |