Article No
LS-C3627-100
AKT3
AKT3 antibody LS-C3627 is an unconjugated rabbit polyclonal antibody to AKT3 (aa119-131) from human. It is reactive with human, mouse, rat and other species. Validated for Func, IP and WB.
Antibodies Primary
Specifications
| NCBI Number | 10000 |
| Application | FA, IP, WB |
| Article No | LS-C3627-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone Type | polyclonal |
| Conjugation | Unconjugated |
| Description | AKT3 antibody LS-C3627 is an unconjugated rabbit polyclonal antibody to AKT3 (aa119-131) from human. It is reactive with human, mouse, rat and other species. Validated for Func, IP and WB. |
| Recommended Dilution | WB (1:500 - 1:2000) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 10000 |
| Gene Symbol | AKT3 |
| Immunogen | KLH-conjugated, synthetic peptide corresponding to aa119-131 (CSPTSQIDNIGEE) of human Akt3/PKBg. The immunizing sequence is identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Hamster, Zebrafish (92%). |
| Isotype | IgG |
| Notes | AKT3 antibody LS-C3627 is an unconjugated rabbit polyclonal antibody to AKT3 (aa119-131) from human. It is reactive with human, mouse, rat and other species. Validated for Func, IP and WB. |
| Alias Names | AKT3, AKT3/PKBgamma, PRKBG, PKB gamma, Protein kinase Akt-3, RAC-gamma, MPPH, PKB-GAMMA, PKBG, Protein kinase B gamma, RAC-PK-gamma, STK-2 |
| Product Type | Antibodies Primary |
| Protocol | Suitable for use in Western Blot and Immunoprecipitation. Western Blot: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates. Immunoprecipitation: 5 ul immunoprecipitated active Akt3/PKBg from 15 nM IGF-I stimulated HEK293 cell lysates. The kinase activity of immunoprecipitated Akt3/PKBg was determined using Akt/SGK Specific Substrate Peptide Active kinase was detected by comparing the incorporation of [32P] into Akt/SGK Substrate Peptide as catalyzed by immunoprecipitates from IGF-I stimulated (15nM) HEK293 cell lysates (1mg). |
| Purification | Affinity Purified |
| Shipping Information | RT |
| Size | 100 µl |
| Source / Host | rabbit |
| Species Reactivity | chicken, cow/bovine, dog/canine, horse/equine, human, mammals, monkey, mouse, pig/porcine, rabbit, rat |
| Storage | -20°C, 4°C |
| Substrate / Buffer | 0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%. |
| Technical Specifications | Recognizes Akt3/PKBg, Mr 60kD. An additional protein was observed, Mr 50kD. Detection of both bands is inhibited by preincubation with the immunizing peptide. Species cross-reactivity: Human. Species sequence homology: mouse and rat. |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
| Volume | 100 µl |
