Article No
LS-C357765-20
NCBI Number | 947 |
Application | FC, IF, IHC, IP, WB |
Article No | LS-C357765-20 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | QBEnd/10 |
Clone Type | monoclonal |
Concentration | 0.2 mg/ml |
Conjugation | Unconjugated |
Description | CD34 antibody LS-C357765 is an unconjugated mouse monoclonal antibody to CD34 (N-Terminus) from human. It is reactive with human, cynomolgus monkey and rhesus monkey. Validated for Flow, IF, IHC, IP and WB. |
Recommended Dilution | FC (0.5 - 1 ug/10e6 cells), IF (0.5 - 1 ug/ml), IHC-P (0.5 - 1 ug/ml), WB (0.25 - 0.5 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 947 |
Gene Symbol | CD34 |
Immunogen | A detergent solubilized vesicular suspension prepared from human term placenta. |
Isotype | IgG1 k |
Notes | CD34 antibody LS-C357765 is an unconjugated mouse monoclonal antibody to CD34 (N-Terminus) from human. It is reactive with human, cynomolgus monkey and rhesus monkey. Validated for Flow, IF, IHC, IP and WB. |
Alias Names | CD34, CD34 molecule, CD34 antigen, HPCA1 |
Product Type | Antibodies Primary |
Protocol | This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85% of angiosarcoma and Kaposi's sarcoma, but with a lower specificity. |
Purification | Protein G Purified |
Shipping Information | RT |
Size | 20 µg |
Source / Host | mouse |
Species Reactivity | human, monkey |
Storage | 4°C |
Substrate / Buffer | PBS, pH 7.4, 0.1 mg/ml BSA, 0.05% Sodium Azide |
Technical Specifications | Human CD34 |
Product Page Updated | 2024-03-11T11:55:05.043Z |