Article No
LS-C357645-100
EPCAM
EPCAM antibody LS-C357645 is an unconjugated mouse monoclonal antibody to human EPCAM. Validated for ELISA and Flow.
Antibodies Primary
Specifications
| NCBI Number | 4072 |
| Application | ELISA, FC |
| Article No | LS-C357645-100 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | ANC8D4 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | EPCAM antibody LS-C357645 is an unconjugated mouse monoclonal antibody to human EPCAM. Validated for ELISA and Flow. |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 4072 |
| Gene Symbol | EPCAM |
| Immunogen | Recombinant human CD326(EpCAM). |
| Isotype | IgG1 k |
| Notes | EPCAM antibody LS-C357645 is an unconjugated mouse monoclonal antibody to human EPCAM. Validated for ELISA and Flow. |
| Alias Names | EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1 |
| Product Type | Antibodies Primary |
| Protocol | Cultured human SW480 cells were harvested by EDTA. Five x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 ul of anti-human CD326 antibody at a concentration of 5 ug/ml. Cells were washed twice and incubated with 50 ul of 2o reagent Goat anti-Mouse Ig/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to Isotype control Mouse IgG1. |
| Purification | Protein A Purified |
| Shipping Information | RT |
| Size | 100 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | 50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl |
| Technical Specifications | Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS. |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
