Article No
LS-C357641-100
NCBI Number | 4072 |
Application | ELISA, FC |
Article No | LS-C357641-100 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | ANC8D4 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | Biotin |
Description | EPCAM antibody LS-C357641 is a biotin-conjugated mouse monoclonal antibody to human EPCAM. Validated for ELISA and Flow. |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 4072 |
Gene Symbol | EPCAM |
Immunogen | Recombinant human CD326(EpCAM). |
Isotype | IgG1 k |
Notes | EPCAM antibody LS-C357641 is a biotin-conjugated mouse monoclonal antibody to human EPCAM. Validated for ELISA and Flow. |
Alias Names | EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1 |
Product Type | Antibodies Primary |
Protocol | Cultured human SW480 cells were harvested by EDTA. Five x 105 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 250 ug/ml (to block non specific binding ) after which they were incubated 45 minutes on ice with 80 ul of anti-human CD326/Biotin at concentration of 5 ug/ml. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.93 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when cells were pre incubated with unlabeled antihuman CD326 mAb (#126-020). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
Purification | Protein A Purified |
Shipping Information | RT |
Size | 100 µg |
Source / Host | mouse |
Species Reactivity | human |
Storage | 4°C |
Substrate / Buffer | 50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA |
Technical Specifications | Antibody ANC8D4 recognizes recombinant human CD326 in EIA, and cell surface human CD326 in FACS. |
Product Page Updated | 2024-03-11T11:55:05.043Z |