Article No
LS-C357637-120
NCBI Number | 29126 |
Application | ELISA, FC |
Article No | LS-C357637-120 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone | ANC6H1 |
Clone Type | monoclonal |
Concentration | 1 mg/ml |
Conjugation | PE |
Description | PD-L1 antibody LS-C357637 is an RPE-conjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for ELISA and Flow. |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 29126 |
Gene Symbol | CD274 |
Immunogen | Recombinant human CD274(B7-H1). |
Isotype | IgG1 k |
Notes | PD-L1 antibody LS-C357637 is an RPE-conjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for ELISA and Flow. |
Alias Names | CD274, B7-H, B7 homolog 1, B7-H1, B7H1, CD274 molecule, CD274 antigen, PDCD1 ligand 1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, Programmed death ligand 1 |
Product Type | Antibodies Primary |
Protocol | Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20^l of 250^g/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ^l of anti-human CD274 at a dilution factor of 1:50 (20 ^g/ml). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ^l of 0.5 mg/ml anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
Purification | Protein A Purified |
Shipping Information | RT |
Size | 120 tst |
Source / Host | mouse |
Species Reactivity | human |
Storage | 4°C |
Substrate / Buffer | 50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA |
Technical Specifications | Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC. |
Product Page Updated | 2024-03-11T11:55:05.043Z |
Volume | 120 tst |