Article No
LS-C357631-120
BTLA / CD272
CD272 antibody LS-C357631 is an APC-conjugated mouse monoclonal antibody to human CD272 (BTLA). Validated for ELISA and Flow.
Antibodies Primary
Specifications
| NCBI Number | 151888 |
| Application | ELISA, FC |
| Article No | LS-C357631-120 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | ANC6E9 |
| Clone Type | monoclonal |
| Conjugation | APC |
| Description | CD272 antibody LS-C357631 is an APC-conjugated mouse monoclonal antibody to human CD272 (BTLA). Validated for ELISA and Flow. |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 151888 |
| Gene Symbol | BTLA |
| Immunogen | Recombinant extracellular human CD272. |
| Isotype | IgG1 k |
| Notes | CD272 antibody LS-C357631 is an APC-conjugated mouse monoclonal antibody to human CD272 (BTLA). Validated for ELISA and Flow. |
| Alias Names | BTLA, BTLA1, B and T lymphocyte associated, CD272, B and T lymphocyte attenuator, B- and T-lymphocyte attenuator, CD272 antigen |
| Product Type | Antibodies Primary |
| Protocol | Five x 105 human peripheral blood mononuclear cells (PBMC) per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD272/APC at a 1:50 dilution (10 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.89 log10 fluorescent units when compared to Mouse IgG1/APC Isotype Control. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD272 antibody. ELISA: Use for capture. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
| Purification | Protein A Purified |
| Shipping Information | RT |
| Size | 120 tst |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | 4°C |
| Substrate / Buffer | 50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA |
| Technical Specifications | Antibody from clone ANC6E9 binds to CD272 on the cell surface of human PBMC in Flow cytometry, and to recombinant CD272-muIg in EIA. |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
| Volume | 120 tst |
