Article No
LS-C347434-0.2
Mouse Anti-Human IgE Secondary Antibody (clone NI 307, Biotin)
Human IgE antibody LS-C347434 is a biotin-conjugated mouse monoclonal antibody. Validated for DB, ELISA, ICC and IHC.
Antibodies Secondary
Specifications
| Application | ELISA, ICC, IHC |
| Article No | LS-C347434-0.2 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | NI 307 |
| Clone Type | monoclonal |
| Conjugation | Biotin |
| Description | Human IgE antibody LS-C347434 is a biotin-conjugated mouse monoclonal antibody. Validated for DB, ELISA, ICC and IHC. |
| Supplier | LifeSpan Biosciences |
| Immunogen | Highly purified polyclonal IgE isolated from human serum. |
| Isotype | IgG1 |
| Notes | Human IgE antibody LS-C347434 is a biotin-conjugated mouse monoclonal antibody. Validated for DB, ELISA, ICC and IHC. |
| Product Type | Antibodies Secondary |
| Protocol | The reactivity of the antiserum is restricted to an isotype specific determinant of the IgE molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno- precipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgE, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. in ELISA from 1:500 upwards. in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only. Working dilutions may vary widely, strongly depending on the test conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. |
| Purification | Purified |
| Shipping Information | RT |
| Size | 0.2 mg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | -20°C, 4°C |
| Substrate / Buffer | Lyophilized from PBS, pH 7.2, No preservatives added |
| Technical Specifications | Human IgE, isotype specific. |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
