Article No
LS-C347204-1
Application | ELISA, ICC, ID, IF, IHC |
Article No | LS-C347204-1 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone Type | polyclonal |
Conjugation | FITC |
Description | Human IgM Fc antibody LS-C347204 is an FITC-conjugated pig polyclonal antibody. Validated for ELISA, ICC, ID, IF and IHC. |
Supplier | LifeSpan Biosciences |
Immunogen | Purified normal IgM isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure. |
Notes | Human IgM Fc antibody LS-C347204 is an FITC-conjugated pig polyclonal antibody. Validated for ELISA, ICC, ID, IF and IHC. |
Product Type | Antibodies Secondary |
Protocol | The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunits of the IgM molecule which expresses strict isotypic specificity. The conjugate does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. |
Purification | Purified |
Shipping Information | RT |
Size | 1 ml |
Source / Host | pig/porcine |
Species Reactivity | human |
Storage | -20°C, 4°C |
Substrate / Buffer | Lyophilized from PBS, pH 7.2, No preservatives added |
Technical Specifications | Human IgM, Fc specific. |
Product Page Updated | 2024-03-11T11:55:05.043Z |
Volume | 1 ml |