The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gel filtration and preparative starch block electrophoresis. In the purification of alpha-2 macroglobulin the activation of kallikrein is avoided, since it forms an irreversible bond with aplpha-2 macroglobulin, thus contaminating the final immunogen. Freund's complete adjuvant is used in the first step of the immunization procedure.
Alpha-2-Macroglobulin antibody LS-C346943 is an unconjugated rabbit polyclonal antibody to mouse Alpha-2-Macroglobulin (A2M ). Validated for ID.
The reactivity of the antiserum is restricted to alpha-2 macroglobulin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified alpha-2 macroglobulin. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence alpha-2 macroglobulin in mouse serum or other body fluids and to determine its concentration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays, but this does not exclude such use if proper controls are included.
Source / Host
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Substrate / Buffer
Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
Mouse Alpha-2 Macroglobulin.
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