Article No
LS-C346490-1
Goat Anti-IgM, Fc Secondary Antibody (FITC)
IgM, Fc antibody LS-C346490 is an FITC-conjugated goat polyclonal antibody. Validated for ELISA, ICC, ID, IF and IHC.
Antibodies Secondary
Specifications
| Application | ELISA, ICC, ID, IF, IHC |
| Article No | LS-C346490-1 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone Type | polyclonal |
| Conjugation | FITC |
| Description | IgM, Fc antibody LS-C346490 is an FITC-conjugated goat polyclonal antibody. Validated for ELISA, ICC, ID, IF and IHC. |
| Supplier | LifeSpan Biosciences |
| Immunogen | Purified IgM isolated from duck serum. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Notes | IgM, Fc antibody LS-C346490 is an FITC-conjugated goat polyclonal antibody. Validated for ELISA, ICC, ID, IF and IHC. |
| Product Type | Antibodies Secondary |
| Protocol | The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against duck serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgM used as immunogens. It does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in duck serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40, depending on the method used. |
| Purification | Purified |
| Shipping Information | RT |
| Size | 1 ml |
| Source / Host | goat |
| Species Reactivity | bird/avian |
| Storage | -20°C, 4°C |
| Substrate / Buffer | Lyophilized from PBS, pH 7.2, No preservatives added |
| Technical Specifications | Duck IgM, Fc specific. |
| Product Page Updated | 2024-04-12T09:20:06.597Z |
| Volume | 1 ml |
