This product is intended for use in precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of alpha-2 macroglobulin in human plasma or other body fluids or to determine its concentration. This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays, but this does exclude such use if proper controls are included.
Source / Host
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Substrate / Buffer
Human A2M / Alpha-2-Macroglobulin. The reactivity of the antiserum is restricted to human alpha-2 macroglobulin. In immunoelectro- phoresis and radial immunodiffusion, using various antiserum concentrations against normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-2 macroglobulin. No reaction is obtained with any other plasma protein components or serum. Cross-reactivity The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has been tested in double radial immunodiffusion against several ser of other species with the following results: bovine + dog t cat + goat - monkey ++ mouse - rat - rabbit +.
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The delivery time for this item is approximately 9-18 business days. If other deviations occur, this will be noted on the order confirmation or communicated via email. Please note, we do reserve the right to select the best packaging and shipping method in order to insure the stability of the product and allow efficient order tracking.