Article No
LS-B2941-50
CD45 / LCA
LCA antibody LS-B2941 is an unconjugated mouse monoclonal antibody to human LCA (CD45) (aa1029-1249). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Antibodies Primary
Specifications
| NCBI Number | 5788 |
| Application | ELISA, IHC, WB |
| Article No | LS-B2941-50 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | 3G4 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | LCA antibody LS-B2941 is an unconjugated mouse monoclonal antibody to human LCA (CD45) (aa1029-1249). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. |
| Recommended Dilution | IHC-P (10 ug/ml), WB (1:1000 - 1:3000) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 5788 |
| Gene Symbol | PTPRC |
| Immunogen | Recombinant human CD45 (1029-1249aa) purified from E. coli. |
| Isotype | IgG2a |
| Notes | LCA antibody LS-B2941 is an unconjugated mouse monoclonal antibody to human LCA (CD45) (aa1029-1249). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. |
| Alias Names | PTPRC, B220, CD45R, CD45, gp180, Leukocyte common antigen, L-CA, Leucocyte common antigen, LY5, T200, T200 glycoprotein, T200 leukocyte common antigen, CD45 antigen, LCA |
| Product Type | Antibodies Primary |
| Protocol | Immunohistochemistry: LS-B2941 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2941 was determined to be 10 ug/ml. |
| Purification | Protein G Purified |
| Shipping Information | RT |
| Size | 50 µl |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | -20°C, 4°C |
| Substrate / Buffer | PBS, pH 7.4, 0.1% Sodium Azide |
| Technical Specifications | Immunohistochemistry: LS-B2941 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2941 was determined to be 10 ug/ml. |
| Product Page Updated | 2024-04-12T06:35:05.844Z |
| Volume | 50 µl |
