Article No
LS-B2596-50
CD40
CD40 antibody LS-B2596 is an unconjugated mouse monoclonal antibody to human CD40. Validated for Flow, IHC and IP. Cited in 1 publication.
Antibodies Primary
Specifications
| NCBI Number | 958 |
| Application | FC, IHC, IP |
| Article No | LS-B2596-50 |
| Country Availability | SE, FI, DK, NO, FO, GL |
| Clone | LOB7/6 |
| Clone Type | monoclonal |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Description | CD40 antibody LS-B2596 is an unconjugated mouse monoclonal antibody to human CD40. Validated for Flow, IHC and IP. Cited in 1 publication. |
| Recommended Dilution | FC (20 ug/ml), IHC-Fr (1 - 10 ug/ml), IHC-P (10 ug/ml) |
| Supplier | LifeSpan Biosciences |
| Entrez Gene ID | 958 |
| Gene Symbol | CD40 |
| Isotype | IgG2a |
| Notes | CD40 antibody LS-B2596 is an unconjugated mouse monoclonal antibody to human CD40. Validated for Flow, IHC and IP. Cited in 1 publication. |
| Alias Names | CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5 |
| Product Type | Antibodies Primary |
| Protocol | Immunohistochemistry: LS-B2596 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2596 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining e. g. pronase or trypsin. Histology Positive Control Tissue: Human Tonsil. |
| Purification | Protein A Purified |
| Shipping Information | RT |
| Size | 50 µg |
| Source / Host | mouse |
| Species Reactivity | human |
| Storage | -20°C |
| Substrate / Buffer | TBS, 0.09% Sodium Azide |
| Technical Specifications | Recognizes the human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions. |
| Product Page Updated | 2024-04-12T06:35:05.844Z |
