Article No
LS-A3520-50
NCBI Number | 5595 |
Application | IHC |
Article No | LS-A3520-50 |
Country Availability | SE, FI, DK, NO, FO, GL |
Clone Type | polyclonal |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
Description | ERK1 antibody LS-A3520 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for IHC. |
Recommended Dilution | IHC-P (7 ug/ml) |
Supplier | LifeSpan Biosciences |
Entrez Gene ID | 5595 |
Gene Symbol | MAPK3 |
Immunogen | Synthetic 18 amino acid peptide from internal region of human MAPK3 / ERK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Panda, Horse, Pig (100%); Bat, Elephant, Rabbit (94%). |
Notes | ERK1 antibody LS-A3520 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for IHC. |
Alias Names | MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, MAP kinase 3, p44-ERK1, ERK1, PRKM3 |
Product Type | Antibodies Primary |
Protocol | Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 7 ug/ml. |
Purification | Affinity Purified |
Shipping Information | RT |
Size | 50 µg |
Source / Host | rabbit |
Species Reactivity | cow/bovine, dog/canine, hamster, horse/equine, human, monkey, mouse, pig/porcine, rat |
Storage | 4°C, -80°C |
Substrate / Buffer | PBS, 0.1% Sodium Azide |
Technical Specifications | Human MAPK3 / ERK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAPK1 (61%). |
Product Page Updated | 2024-04-12T06:35:05.844Z |
Volume | 50 µl |